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the gap involving the oxygen atom of Thr164 of Aph1 along with the oxygen atom of Tyr466 of PS1 is predicted to get three.5 Å, suggesting that a hydrogen bond (H...O distance, roughly one.8 Å) is formed in these aspect chains (determine 7b). The T164A mutation might get rid of the hydrogen bond with PS1 Y466, leading to flexible motion of the carboxy terminus of PS1. We suggest that Aph1 mutations regulate protease activity by conformational changes in PS1.
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